Final Project Reflective Paper - Team Beta Assignment

Final Project Reflective Paper - Team Beta - Assignment Example Consequently, it is a suitable platform for human resource managers to evaluate their progress and effect adjustment in their human resource management strategies. Management Introduction While implementing projects in new environments, any organization will require to strategize on how to manage its resources including the human labour in an effort to penetrate the market and compete with the external and internal market forces. The human labour is a crucial element of any business, and its management and effective utilization has a direct impact on the organization’s success. The productivity of human labour in all the phases of project implementation lies on the shoulders of the organizational managers. The role of human resource manager in an organization is to recruit, train, monitor, evaluate and motivate the employees during the project implementation process. The performance of an organization will depend on its ability to exercise effective organization behavior and s kills while managing teamwork among the role players within the system. To do this, a company will require to conduct a SWOT analysis as a strategy of re-evaluating its effectiveness in gathering its strengths, neutralizing its threats and weaknesses and grasping its opportunities presented in the environment. Effective team management and organizational behavior are the basic elements that the human resource management must possess in order to survive in the diverse market. Just like any other organization, Team Beta has faced the challenge of managing the team players during the implementation of its performance based scenario planning project. To maintain teamwork within the four members who have a variant cultural background is a credit worth effort that requires effective management skills that can stand the tests of a harsh project environment. The purpose of this article is engage in a critical reflective practice that Brookfield (1998) terms as an effective procedure to chec k the success of any team leader at each stage of a project. Organizational Behaviors and Skills Required in a Successful 21st-Century Organizational transformation is an element of business that an organization cannot ignore in the 21st century. With the proliferation of technology the barriers of business boundaries have become a thing of the past and the world has condensed into a small village. This is a century that has been dominated by trade globalization and extension of business boundaries to feature international business markets (Kreitman, 1997). These changes have had a great impact on trade and organizations are under the pressure to readjust their organizational behavior to suit diversified business environments that are characterized with unpredictable market forces. In team management, it is important to adopt a behavior that suits the modern approach of team management. One challenge that arises team management is the understanding of the available labour force, its management and motivation of the role players. Global diversity management advocates that team leaders provide opportunities for each employee to grow and develop within the organizations that they work in. It requires that the organization takes

Functional Relationship Network Architecture

Functional Relationship Network Architecture A computer network, is referred to as a network, it is a harvest of computers and instruments interconnected via communication channels that enables communications among users and permits users to allocated resources. Networks may be classified according to a wide range of characteristics. A computer network permits sharing of resources and knowledge among interconnected devices. Fig1:Block diagram of computer network Connection method Computer networks can be classified according to the hardware and software engineering that is accustomed to interconnect the individual devices in the network, such as optical fiber, Ethernet, wireless LAN. Functional relationship (network architecture) Computer networks may be classified according to the functional relationships which exist among the elements of the network, e.g., active networking, client-server and peer-to-peer architecture. Network topology Computer networks may be classified according to the network topology upon which the network is grounded, such as bus network, star network, ring network, mesh network. Network topology is the coordination by which tools in the network are organized in their rational family members to one another, independent of physical arrangement. Even if networked computers are physically placed in a linear arrangement and are joined combined to a hub, the network has a star topology, alternatively a bus topology. In this regard the visual and operational aspects of a network are distinct. Networks may be classified grounded on the process of knowledge adapted to carry the data; these include digital and analog networks. Fig2. Mesh topology Fig3. Star Topology Fig4. Ring topology What is a firewall? Fig5. firewall A firewall is a component of a computer system or network that is arranged to avoid unauthorized access where letting agent communications. It is a implement or set of tools that is configured to sanction or turn down network transmissions grounded upon a set of administers and other criteria. Firewalls can be implemented in either hardware or software, or a combination of two. Firewalls are commonly adapted to prevent unauthorized Internet users from accessing private networks joined combined to the Internet, especially intranets. All messages entering or withdrawing the intranet surpass through the firewall, which inspects each outcome and prevents those that do not find the specified protection criteria. There are several types of firewall techniques: Packet filter: Packet filtering checks each packet that is passing through the network and accepts or refuses it based on particular IP addresses that is user defined. Although difficult to configure, it is effective and mostly transparent to its users. It is vulnerable to Internet Protocol spoofing. Fig6. Packet filters This type of packet filtering pays no heed to if a packet is part of an older stream of traffic (i.e. it stores no information on connection state). Instead, it filters each packet based only on information contained in the packet itself . TCP and UDP protocols consists most communication over the net, and because TCP and UDP traffic by convention uses well known ports for some types of traffic, a stateless packet filter can differentiate between, and hence control, those types of traffic (such as web browsing, remote printing, email transmission, file transfer), untill the machines on each side of the packet filter are both using the same non-standard ports. Packet filtering firewalls work mainly on the initial three layers of the OSI reference model, which means most of the work is done in between the network and physical layers, with a little bit of peeking into the transport layer to find out source and destination port numbers. When a packet originates from the sender and filters through a firewall, the device finds matches to any of the packet filtering rules that are configured in the firewall and removes or rejects the packet accordingly. When the packet goes through the firewall, it checks the packet on a protocol/port number basis (GSS). Application gateway: Applies security mechanisms to some applications, such as FTP server. This is effective, but can degrade the performance Fig7.OSI reference model The benefit of application layer filtering is that it can understand applications and protocols and it can also detect if an unwanted protocol is sneaking through on a non-standard port or if a protocol is being used in any harmful way. An application firewall more secure and reliable as compared to packet filter firewalls as it works on all 7 layers of the OSI reference model, from the application to the physical layer. This is similar to a packet filter firewall but here it also filters information on the basis of content. In 2009/2010 the focus of the best comprehensive firewall security vendors turned to expanding the list of applications such firewalls are aware of now covering hundreds and in some cases thousands of applications which can be identified automatically. Many of these applications can not only be blocked or allowed but copied by the more advanced firewall products to allow only certain functionally enabling network security administrations to give users functionality without enabling unnecessary vulnerabilities. As a consequence these advanced versions of the Second Generation firewalls are being referred to as Next Generation and bypass the Third Generation firewall. It is expected that due to malicious communications this trend will have to continue to enable organizations to be truly secure. Third generation: stateful filters Fig8. Stateful filter Third-generation firewalls, in addition to what first- and second-generation look for, regard placement of each packet within the packet series. This technology is generally referred to as a stateful packet inspection as it maintains records of all connections going through the firewall and is able to determine whether a packet is the start of a new connection, a part of an existing connection, or is an invalid packet. Though there is still a set of defined rules in such a firewall, the state of a connection can itself be one of the criteria which trigger specific rules. This type of firewall can actually be exploited by certain Denial-of-service attacks which can fill the connections with illegitimate connections. Circuit-level gateway: Applies security mechanisms when a TCP or UDP connection is established. Once the connection has been done, packets can go between the hosts without checking further. Stateful filters Fig8. Stateful filter Third-generation firewalls, in addition to what first- and second-generation look for, regard placement of each packet within the packet series. This technology is referred to as a stateful packet inspection as it maintains records of all connections going through the firewall and is able to determine whether a packet is the start of a new connection, a part of an existing connection, or is an invalid packet. Though there is still a set of static rules in such a firewall, the state of a connection can itself be one of the criteria which trigger specific rules. This type of firewall can actually be abused by some Denial-of-service attacks which can fill the connection tables with false connections. Proxy servers Checks all messages entering and leaving the network. The proxy server hides the right network addresses. Fig9.Proxy server In computer networks, a proxy server is a server that acts as an intermediary for requests from clients seeking resources from other servers. A client connects to the proxy server, asking for some service, such as a file, connection, web page, or other resource, available from a different server. The proxy server processes the request according to its filtering rules. For example, it may filter traffic by IP address. If the request is passed by the filter, the proxy provides the resource by connecting to the relevant server and requesting the service on behalf of the client. A proxy server may alter the clients request or the servers response, and sometimes it may pass the request without contacting the specified server. In this case, it caches responses from the remote server, and sends back subsequent requests for the same content directly. Types of proxy Forward proxies Fig10.Forward proxies A forward proxy taking requests from an internal network and forwarding them to the Internet. Forward proxies are proxies where the client server names the target server to connect to. Forward proxies are able to get from a wide range of sources. The terms forward proxy and forwarding proxy are a general description of behavior (forwarding traffic) and hence ambiguous. Except for Reverse proxy, the types of proxies described on this article are more specialized sub-types of the general forward proxy concepts. Open proxies Fig11.Open proxies An open proxy forwarding requests from and to anywhere on the Internet. An open proxy is a forward proxy server that is accessible by any Internet user. Gordon Lyon estimates there are hundreds of thousands of open proxies on the Internet. An anonymous open proxy allows users to conceal their IP address while browsing the Web or using other Internet services. Reverse proxies Fig12.Reverse proxies A reverse proxy taking requests from the Internet and forwarding them to servers in an internal network. Those making requests connect to the proxy and may not be aware of the internal network. A reverse proxy is a proxy server that appears to clients to be an ordinary server. Requests are forwarded to one or more origin servers which handle the request. The response is returned as if it came directly from the proxy server. Reverse proxies are installed in the neighborhood of one or more web servers. All traffic coming from the Internet and with a destination of one of the web servers goes through the proxy server. The use of reverse originates in its counterpart forward proxy since the reverse proxy sits closer to the web server and serves only a restricted set of websites. There are several reasons for installing reverse proxy servers: Encryption / SSL acceleration: when secure web sites are created, the SSL encryption is often not done by the web server itself, but by a reverse proxy that is equipped with SSL acceleration hardware. See Secure Sockets Layer. Furthermore, a host can provide a single SSL proxy to provide SSL encryption for an arbitrary number of hosts; removing the need for a separate SSL Server Certificate for each host, with the downside that all hosts behind the SSL proxy have to share a common DNS name or IP address for SSL connections. This problem can partly be overcome by using the SubjectAltName feature of X.509 certificates. Load balancing: the reverse proxy can distribute the load to several web servers, each web server serving its own application area. In such a case, the reverse proxy may need to rewrite the URLs in each web page (translation from externally known URLs to the internal locations). Serve/cache static content: A reverse proxy can offload the web servers by caching static content like pictures and other static graphical content. Compression: the proxy server can optimize and compress the content to speed up the load time. Spoon feeding: reduces resource usage caused by slow clients on the web servers by caching the content the web server sent and slowly spoon feeding it to the client. This especially benefits dynamically generated pages. Security: the proxy server is an additional layer of defense and can protect against some OS and Web Server specific attacks. However, it does not provide any protection to attacks against the web application or service itself, which is generally considered the larger threat. Extranet Publishing: a reverse proxy server facing the Internet can be used to communicate to a firewalled server internal to an organization, providing extranet access to some functions while keeping the servers behind the firewalls. If used in this way, security measures should be considered to protect the rest of your infrastructure in case this server is compromised, as its web application is exposed to attack from the Internet. VPN A virtual private network (VPN) is a computer network that uses a public telecommunication infrastructure such as the Internet to provide remote offices or individual users with secure access to their organizations network. It aims to avoid an expensive system of owned or leased lines that can be used by only one organization. It encapsulates data transfers between two or more networked devices which are not on the same private network so as to keep the transferred data private from other devices on one or more intervening local or wide area networks. There are many different classifications, implementations, and uses for VPNs. Fig13 VPN Vulnerabilities:- Unauthorized access: This simply means that people who shouldnt use your computer services are able to connect and use them. For example, people outside your company might try to connect to your company accounting machine or to your network file server. There are various ways to avoid this attack by carefully specifying who can gain access through these services. You can prevent network access to all except the intended users. Exploitation of known weaknesses: Some programs and network services were not originally designed with strong security in mind and are inherently vulnerable to attack. The BSD remote services (rlogin, rexec, etc.) are an example. The best way to protect yourself against this type of attack is to disable any vulnerable services or find alternatives. With Open Source, it is sometimes possible to repair the weaknesses in the software. Denial of service: Denial of service attacks cause the service or program to cease functioning or prevent others from making use of the service or program. These may be performed at the network layer by sending carefully crafted and malicious datagrams that cause network connections to fail. They may also be performed at the application layer, where carefully crafted application commands are given to a program that cause it to become extremely busy or stop functioning. Preventing suspicious network traffic from reaching your hosts and preventing suspicious program commands and requests are the best ways of minimizing the risk of a denial of service attack. Its useful to know the details of the attack method, so you should educate yourself about each new attack as it gets publicized. Spoofing: This type of attack causes a host or application to mimic the actions of another. Typically the attacker pretends to be an innocent host by following IP addresses in network packets. For example, a well-documented exploit of the BSD rlogin service can use this method to mimic a TCP connection from another host by guessing TCP sequence numbers. To protect against this type of attack, verify the authenticity of datagrams and commands. Prevent datagram routing with invalid source addresses. Introduce unpredictability into connection control mechanisms, such as TCP sequence numbers and the allocation of dynamic port addresses. Eavesdropping: This is the simplest type of attack. A host is configured to listen to and capture data not belonging to it. Carefully written eavesdropping programs can take usernames and passwords from user login network connections. Broadcast networks like Ethernet are especially vulnerable to this type of attack Here are a few examples of firewalls :- Untangle Fortiguard Netnanny Websense ClearOS These firewalls can be affected by the above vulnerabilities. One way how a firewall/web filter can be bypassed is by using VPN. As studied above we can VPN to some external network and use that network. So we can bypass the firewall by doing VPN to a remote network and using its default gateway. Below are the precise steps how to setup a VPN server, Client, AD and LB configurations. Complete VPN Configuration Below is the complete procedure on how to setup VPN server and client side Note:- Windows XP and Windows 7 both have the capability to act as VPN servers VPN Server Configuration Open Network connections and follow the below :- Click next on the welcome page Select the options highlighted in the below snags :- Once you have followed the steps above you are done with the server side configuration. VPN Client Configuration Below snags show the client side configuration Once the above steps are followed the client side is also setup The work is still not over Port Forward Port needs to be forwarded from the modem/LB etc Follow the instructions below to get it rolling :- Dial in Rights on AD The final step is to give the user permissions to VPN First RDP to the AD Login Open Active Directory Find the user and go in properties Follow the snag it once the above is done :- The best firewall:- According to the first hand experience we found Untangle to be the best firewall as it is free and has a host of functions too. Below is a screenshot of the untangle dashboard:- Fig14. Untangle dashboard Conclusion:- Our aim was to explain what a firewall is and expose a few vulnerabilities in it. We have studied how a firewall works, its architecture, types of firewalls and vulnerabilities. We have thus compared the firewalls on various parameters and have concluded that Untangle is the best firewall with reference to the features and cost of it.

Roll of Thunder, Hear My Cry by Mildred D. Taylor is a very powerful Es

Roll of Thunder, Hear My Cry by Mildred D. Taylor is a very powerful novel about the Logan family living in Mississippi in the 1930's. Roll of Thunder, Hear My Cry by Mildred D. Taylor is a very powerful novel about the Logan family living in Mississippi in the 1930's. The story is told through the eyes of nine-year-old Cassie Logan, who has to learn throughout the entire book, how harsh the world truly is for blacks. Cassie has to learn that it is a white man's world out there, and that black people don't have much of a say in what goes on in it. There are many events in this book which lead me to say how prejudiced our country was at that time. One thing that stays on my mind is the conflict between Cassie and Lillian Jean, a white girl about the same age as Cassie. During Cassie's first visit to the town of Strawberry, she accidentally bumps into Lillian Jean. It is then that Lillian Jean starts calling Cassie names (comparing Cassie's "nasty little self" with "decent white folk"), and makes her apologize. Big Ma and Charlie Simms, Lillian Jean's father, force Cassie to do so, and Cassie is also told to call her, "Miz" Lillian Jean. Cassie does not understand why this must be so, and the idea of calling someone "Miz", who is the same age as herself, hits Cassie hard. She takes her revenge by beating Lillian Jean up, and also blackmailing her. It is the first time that Cassie realizes that white and black children are treated differently. It shows how black children are forced to feel inferior to white children. Another cause of racism is where the whites hate the Logan family for owning their own land. Throughout the book, there are many efforts from the white people- mainly, Mr. Granger- to get... ...is saved for the moment, though because of the fact that he is black, it is unlikely that T.J. will be saved. This situation just goes to show that the idea that all men are created equal in the U.S., does not apply to the blacks in Roll of Thunder. I can see from Mildred D. Taylor's Roll of Thunder, Hear My Cry, that racism affected the black people in the Deep South, years after slavery was abolished. Everywhere you turn, you can see that this issue was a daily part of their lives. The law turned a blind eye to crimes committed against blacks. The education system for blacks was second-rate. Black children were separated from whites from the beginning, thus making the blacks hate whites, as they grew older. Whites did not want blacks to feel in any way equal to them. And from the book, Roll of Thunder, Hear My Cry, I can better understand this issue. Roll of Thunder, Hear My Cry by Mildred D. Taylor is a very powerful Es Roll of Thunder, Hear My Cry by Mildred D. Taylor is a very powerful novel about the Logan family living in Mississippi in the 1930's. Roll of Thunder, Hear My Cry by Mildred D. Taylor is a very powerful novel about the Logan family living in Mississippi in the 1930's. The story is told through the eyes of nine-year-old Cassie Logan, who has to learn throughout the entire book, how harsh the world truly is for blacks. Cassie has to learn that it is a white man's world out there, and that black people don't have much of a say in what goes on in it. There are many events in this book which lead me to say how prejudiced our country was at that time. One thing that stays on my mind is the conflict between Cassie and Lillian Jean, a white girl about the same age as Cassie. During Cassie's first visit to the town of Strawberry, she accidentally bumps into Lillian Jean. It is then that Lillian Jean starts calling Cassie names (comparing Cassie's "nasty little self" with "decent white folk"), and makes her apologize. Big Ma and Charlie Simms, Lillian Jean's father, force Cassie to do so, and Cassie is also told to call her, "Miz" Lillian Jean. Cassie does not understand why this must be so, and the idea of calling someone "Miz", who is the same age as herself, hits Cassie hard. She takes her revenge by beating Lillian Jean up, and also blackmailing her. It is the first time that Cassie realizes that white and black children are treated differently. It shows how black children are forced to feel inferior to white children. Another cause of racism is where the whites hate the Logan family for owning their own land. Throughout the book, there are many efforts from the white people- mainly, Mr. Granger- to get... ...is saved for the moment, though because of the fact that he is black, it is unlikely that T.J. will be saved. This situation just goes to show that the idea that all men are created equal in the U.S., does not apply to the blacks in Roll of Thunder. I can see from Mildred D. Taylor's Roll of Thunder, Hear My Cry, that racism affected the black people in the Deep South, years after slavery was abolished. Everywhere you turn, you can see that this issue was a daily part of their lives. The law turned a blind eye to crimes committed against blacks. The education system for blacks was second-rate. Black children were separated from whites from the beginning, thus making the blacks hate whites, as they grew older. Whites did not want blacks to feel in any way equal to them. And from the book, Roll of Thunder, Hear My Cry, I can better understand this issue.

Discuss how Stevenson presents duality in ‘Dr Jekyll and Mr Hyde Essay

In this essay I will show how Robert Louis Stevenson has presented duality in his novella ‘Dr Jekyll and Mr Hyde’. The novella is about a respectable gentleman, Dr Jekyll, and how, under the pressure of high society in Victorian England, experiments with potions to eventually come up with one that would turn him into Mr Hyde, a disreputable and evil man. Written in 1886, the novella was based around the pressure to be respectable that Robert Louis Stevenson himself felt in high society of the Victorian era. It was also influenced by scandals of the time such as Deacon Brodie. Brodie, who suffered from gambling debts, was a cabinet maker for people in the higher class. To try and pay off his debts he would break into the cabinets that he had sold and steal the valuables inside. This fits into the story of Jekyll and Hyde since Jekyll is a nice, respectable gentleman who turns into an evil, lower class man, Hyde. There was also a growing awareness of chemistry and psychology at the time the novella was written. Sigmund Freud, a famous chemist and psychologist, convinced people that duality did exist in humans – that in one person there could be both good and evil, such as in Jekyll and Hyde, who were the same person, with the help of a potion, but Jekyll was good and Hyde evil. Since the novella was written in 1886 it was targeted at Victorian people. When it was first published it sold around 40,000 copies, mainly to the higher classes of Victorian England. They would have seen it as a twist on a horror book. The Victorians were into gothic books, except that they were always set in foreign countries and in the past. ‘Dr Jekyll and Mr Hyde’ differs to these stories since it was set in London and in the then present day. In 1888, Jack the Ripper surfaced in the newspapers. This would not only have boosted the sales of Stevenson’s novella but would have been connected to it. Both the former and the latter ideas are because of the rumour that went around England at the time that Jack the Ripper was someone of upper class and respectable by day but commit atrocious murders by night, like the duality of Jekyll and Hyde. Duality appears throughout the novella, including the characters. Mr Utterson is a lawyer and good friend of Henry Jekyll. â€Å"†¦of a rugged countenance, that was never lighted by a smile; cold, scanty†¦Ã¢â‚¬  This description of Mr Utterson, from the first page, portrays him to be a grumpy man, with sharp features on his face, who doesn’t get on with anybody and who would drink on his own. However: â€Å"†¦somehow loveable.† This quote, again from the first page, shows the duality of the book since Utterson is described to be miserable and yet everybody loves him. This is enforced by the adjective ‘lovable’ since this word implies that he is pretty easy to get on with and he isn’t just liked by those who know and get on with him, instead he is loved. The quotes convey that no matter what someone’s demeanour they can still be kind and popular. This introduces the theme of duality for the reasons said above. â€Å"I let my brother go to the devil in his own way.† Being a lawyer, Mr Utterson is supposed to help others in any way he can, however, this metaphor is telling the reader that no matter how much he could do for someone, Utterson doesn’t really care about them. This portrays Robert Louis Stevenson’s idea of duality. â€Å"†¦least save his credit†¦I shall be back before midnight, when we shall send for the police.† This quote shows duality since Utterson himself said that he would ‘let his brother go to the devil in his own way’, yet here he is trying to save the reputation of one of his very good friends, Jekyll. To do this though, the good, honourable, respectable, law abiding lawyer does not send for the police as soon as he and Poole, Dr Jekyll’s butler, discover the dead body of a certain Mr Edward Hyde lying on Jekyll’s cabinet floor. Instead, he goes home for two hours to read the letters that both Dr Lanyon, another good friend of both Utterson and Jekyll, and Jekyll left for him to read on the disappearance or death of Henry Jekyll. It also shows duality in that Utterson is a lawyer, who should go to the police but doesn’t. â€Å"†¦in case of disappearance†¦read the name Gabriel John Utterson.† This quote shows duality because as Utterson finds, and reads, Jekyll’s Last Will and Testament, Utterson, to his own amazement, reads his name instead of Hyde’s. This shows duality since Utterson is down as the last good friend of Dr Henry Jekyll, who would become disreputable and a big scandal if any one were to find out what he did. Dr Lanyon is another character in the novella, and a good friend of Jekyll and Utterson. â€Å"This was a hearty, healthy, dapper, red faced gentleman with a shock of hair prematurely white.† This quote portrays Lanyon to be a friendly, upper class gentleman who has plenty to drink. However, later on in the novella, Stevenson describes Lanyon: â€Å"The rosy man had grown pale; his flesh had fallen away; he was visibly balder and older†¦Ã¢â‚¬  This quote and the latter, reveals the duality between them since in the first quote he is described as being healthy and in the second as being on his death bed. The reader would want to know what has happened in such a short time to make this change in Lanyon appear so suddenly – because he saw Hyde mix the potion, take a drink, and turn to Jekyll in front of his very own eyes – all of which is revealed in the second to last chapter ‘Dr Lanyon’s Narrative’. To get the potions to Hyde however, Jekyll had to get Lanyon to steal for him. The very idea of Jekyll wanting another respectable gentleman breaking in and stealing the potions for him and Hyde would have been a very big scandal if Lanyon was caught, and Lanyon would go from respectable gentleman to disrespectable in a few hours. Stevenson’s novella is all about Dr Jekyll and Mr Hyde. â€Å"†¦the doctor gave one of his pleasant dinners†¦Ã¢â‚¬  This shows how Jekyll is popular among the upper class, among his friends, and rich enough to hold dinners. The adverb ‘pleasant’ conveys to the audience that everyone Jekyll invited got on with each other and it reinforces the idea that Jekyll is rich since he can hold dinners and provide nice food. â€Å"†¦sat Dr Jekyll, looking deadly sick.† This quote describes Jekyll sitting in his large cabinet room, after the death of sir Danvers Carew. It reveals how Jekyll is feeling remorse for knowing, or as the reader later finds out, for being Mr Hyde – who was identified as the murderer of Carew. It portrays how Jekyll regrets his actions and wishes, to get away from the pressures of high society life in Victorian England. â€Å"†¦8th of January Utterson had dined at the doctors†¦On the 12th, and again on the 14th, the door was shut against the lawyer.† This reference conveys to the audience the duality in the novella, since four days after Utterson had seen Jekyll, and dined with him and Dr Lanyon, he was being denied entry to Jekyll’s house. This shows the duality since Jekyll was so ill that he couldn’t stand up to greet Utterson when Utterson went to see him, before being fine and healthy enough to hold a dinner party and then not allowing anyone into his house to see him all of a sudden without anybody knowing why. â€Å"†¦born in 18__ to a large fortune†¦Ã¢â‚¬  This quote portrays how Henry Jekyll recognises how he was born into a rich family. The adjectives ‘large’ and ‘fortune’ suggests he was born into a highly respected family, something that was of high importance in Victorian England. It shows how he didn’t have to work hard for the position in society that he was in, only keep up his appearances with others of his class. â€Å"†¦worst of my faults was a certain gaiety of disposition, such as made the happiness of many†¦Ã¢â‚¬  This reference shows that Jekyll felt money wasn’t everything. He felt that where it made most men content, it didn’t make him happy. It conveys to the reader how he wants to be happy, although where he is in life and society wasn’t making his wishes come true. We learn later in the novella how this wish brings him to start mixing formulas that would eventually turn him into Mr Hyde. â€Å"†¦found it hard to reconcile with my imperious desire to carry my head high†¦Ã¢â‚¬  The quote above portrays how, although Jekyll wants to be happy, he believes the only way would be one that was irreputable. However, he does not wish to lose his place in the upper class of society and he does not wish to lose his friends, both of which would happen if he did what he desired to do to become happier. This therefore is what led Jekyll to create the potion, as well as the written version of events, for Utterson to read, in ‘Henry Jekyll’s full statement of the case’ where Dr Jekyll writes his version, and the truth of what happened in the last months of his life. â€Å"Hence it came about that I concealed my pleasures†¦Ã¢â‚¬  This reveals how Jekyll had been hiding secrets since before creating the mixture that would turn him to Hyde, hiding what it was that was making him happy. The verb ‘concealed’ tells the reader that Jekyll was being very careful about his pleasures. It conveys the importance of nobody finding out about Jekyll’s secret more than if Stevenson had written the verb ‘hid’ instead. â€Å"†¦already committed to a profound duplicity of life.† This conveys one of the themes in the novella, the theme of duplicity, and how it is not only in the settings and the characters but that the characters knew about it. We know this because of the adjective ‘duplicity’ – portraying to the reader how Jekyll has two lives, however different they are. â€Å"†¦morbid sense of shame.† This quote reveals how although Jekyll wanted to be happy, he is ashamed of how his life has turned out. The alliteration of the ‘s’ sound in ‘sense’ and ‘shame’ enforces the idea, in the readers mind, that he is ashamed of being Mr Hyde, of what he has done and is still doing as Mr Hyde and that both of these irreputable things are making him happy. â€Å"†¦not truly one, but truly two.† This does not reveal duality within the story, rather in the themes of the novella. It indicates the views of Dr Jekyll – that in one man, there is both good and evil, one of the themes. Jekyll describes how good and evil are different parts of the soul, and that good conquers evil in a raging war within the soul, and that is what makes a man good. â€Å"†¦flushed as I was with hope and triumph, to venture in my new shape†¦Ã¢â‚¬  As this quote conveys, Stevenson has written about how someone can be addicted to drugs. It shows that even someone of high stature can become addicted, in this case Dr Jekyll. Jekyll is addicted to how he can do what he wants as Mr Hyde, without losing any of his own stature, rather than do the respectable things he would have to do as himself to be happy. It reveals duality in the fact that a respectable gentleman such as Jekyll can be addicted to the painful pangs and nausea the mixture makes him feel, whilst turning into Hyde. Edward Hyde is often portrayed animal-like. â€Å"†¦like a monkey jumped up from among the chemicals.† This quote makes the reader imagine a monkey like creature jumping up upon hearing Poole, Jekyll’s butler, coming towards Jekyll’s cabinet. It creates the picture of Hyde being small, dumpy, and hairy and as having very long arms, whilst showing duality since Hyde is a man not a monkey. â€Å"The other snarled aloud into a savage laugh†¦Ã¢â‚¬  In this quote the adjective ‘snarled’ gives the image of Hyde as a savage beast, again being portrayed as some sort of animal. It also conveys how Hyde is an evil person, since someone who is portrayed as being a savage cannot be any good, and this would have been the view of people in Victorian England. Stevenson has used language to paint a picture of what Hyde might look like in the readers mind. â€Å"†¦so ugly that it brought out the sweat on me like running.† This reference, again, allows the reader to imagine what Hyde looks like. It conveys how the other characters react to Hyde, that they all feel an air of deformity whenever they see him or speak to him, and that he looks so ugly, so mean that they feel uneasy around him. All of the latter three quotes show duality since Hyde is portrayed as being a small and ugly man with the impression of being deformed somewhere on his body. He is also conveyed as a mean, evil person via the descriptive language used by Stevenson. This is in contrast to Jekyll, since Jekyll is a respected gentleman and doctor of chemistry, who is regularly invited to dinner parties hosted by other well respected people in society. He is also taller, thinner and older than Hyde. All of the characters who ‘meet’ Hyde in the novella seem to act in the same way towards him. This conveys to the reader just how unlikeable Hyde is. This is important to the story because it shows how everybody thinks him an evil man. It helps show the duality between Jekyll and Hyde. However, it isn’t just the characters that show duality within Stevenson’s â€Å"Dr Jekyll and Mr Hyde†. It is also revealed in the settings – the weather and the buildings – and also in the narrative structure. â€Å"The door†¦neither bell nor knocker, was blistered and distained. Tramps slouched†¦Ã¢â‚¬  This quote portrays the quality of the door that Hyde uses to enter Dr Jekyll’s house and grounds. This is in contrast to the front of Jekyll’s house: â€Å"†¦which wore a great air of wealth and comfort†¦Ã¢â‚¬  This portrays an image of what Jekyll’s house looks like from the front – a grand house whose owner is wealthy and of a high social class. The latter quote also shows duality with Hyde’s house in Soho. â€Å"†¦showed him a dingy street†¦Ã¢â‚¬  This quote portrays what kind of area Hyde’s house is in. In the Victorian era, Soho was poverty stricken and full of prostitution, although there is no indication that Hyde was into prostitution and gambling (other than Jekyll turning into Hyde because doing respectable things did not make him happy). It shows the difference between Jekyll’s big, respectable house in a respectable of London and Hyde’s dingy flat on a polluted street in the centre of London. â€Å"An ivory faced and silvery haired woman†¦Ã¢â‚¬  This quote describes the Soho house landlady (or Hyde’s landlady). When the reader reads this they presume she is a nice friendly woman. However: â€Å"She had a smooth face, smoothed by hypocracy†¦Ã¢â‚¬  shows duality in contrast with the quote before. It shows how a nice old lady may look nice but in fact can be evil, someone who doesn’t like herself. I say evil because later in the chapter ‘The Carew Murder Case’ she is excited and delighted by the idea of Hyde being in trouble with the police. Hyde’s house in Soho is, to Utterson’s surprise, well furnished. â€Å"†¦furnished with luxury and good taste†¦Ã¢â‚¬  This conveys duality with how the house looks on the outside. With Soho being a dodgy area in Victorian England, and the street being described as ‘dingy’, the reader first imagines a poor, poverty filled room, not a luxury, well furnished house. London is also shown in a dual nature. â€Å"†¦down a by street in a busy quarter of London. The street was small and what is called quiet.† This quote shows duality in how although a street is in a busy area of London, off a busy main street; it is actually very quiet (it would be expected to be busy if it’s off a main street). Stevenson has even included duality in the weather. â€Å"†¦cool and a little damp, and full with premature twilight,†¦still bright with sunset.† This quote conveys duality to the reader since Jekyll’s courtyard is described at the beginning of the quote – that it is cold and looks as though it is around the time frame of dusk. However, the later half of the quote explains, it is in fact still sunny (nearing sunset) and so in theory Jekyll’s courtyard should be quite light. â€Å"A fog rolled over the city†¦ early part of the night was cloudless.† This reveals duality because it shows how the night was cloudless, until the fog came in. the adjective ‘rolled’ gives the impression that the fog came swiftly, rather than slowly. â€Å"About nine in the morning†¦ number of degrees and hues of twilight†¦ dark like the back end of evening†¦Ã¢â‚¬  This quote conveys to the reader that whatever time of day it is in Soho, it still looks like it’s dark, like it is night. This quote shows the duality of the weather by giving the time of day and describing what it looked like. The fog described is more likely to be smog from the factories, since the novella is set in the Victorian times. However, it does cast an eerie effect on the image conjured in the readers mind, would have made them think something sinister was about to happen. There are many locked doors in Stevenson’s novella. This symbolises how secretive the story is, Utterson hypothetically being stopped solving the mystery of Jekyll and Hyde, by doors not able to be opened until another section of the mystery is found, and the actual looked doors that Jekyll shuts himself up behind. The narrative structure also shows duality since in the first seven chapters the narrative is third person. â€Å"†¦resumed the lawyer.† This shows the third person narrative structure of ‘The Last Night’. However, the last two chapters are written in first person, ‘Dr Lanyon’s Narrative’ is written by Lanyon from his point of view and tells of what he knows about Jekyll and Hyde, and explains the cause of his death. The last chapter is also in first person, however this is from the perspective of Dr Jekyll himself, who explains everything that had happened. â€Å"I rose from my place†¦Ã¢â‚¬  These show duality because the first seven chapters, although written in third person, are all about Utterson and what he does to try and unravel the mystery between Jekyll and Hyde. They also show Utterson’s thoughts and feelings. The chapters are all arranged to follow what happens to Utterson and the titles are all to do with what happens within the chapter itself (and give a clue to what the chapter is about). The last two are in the order they are because that way Lanyon doesn’t repeat what the reader knows from reading Jekyll’s chapter, if they were the other way around. In this essay I have shown how Robert Louis Stevenson has presented the theme of duality in his novella ‘Dr Jekyll and Mr Hyde’. I have achieved this by analysing the language of the text that describes the characters, weather, buildings and the narrative structure.

Existentialism and Human Nature Essay

Absolute individuality and absolute freedom: the basis of all existentialist arguments. The existentialist’s conceptions arise from their held views that since we are all ultimately alone, we have absolute freedom over our nature. Existentialists emphasize the â€Å"free and conscious self† which opponents constantly attack, exclaiming that there is a â€Å"higher power† enabling our consciousness. But are humans so simple? Can things be explained solely on blaming ourselves or another being for our nature? The existentialist generally believes in a sole existence; meaning that we are alone in the world, and that we have no one but ourselves. They also believe in the human being’s capacity to feel more than one kind of pain, and that there are three different levels to our pain, physical, psychological, and emotional. All of these types of pain can cause us to become irrational and anxious. But are we really anxious because of this pain, or is it because we ultimately feel alone? And if we are all individuals, can we really feel the same kind of pain as someone else? Existence precedes essence is also dominant in the mind and thoughts of the existentialist. That is, the idea that we are born into this world without a predetermined nature, and only later in life do we shape out nature through actions and choices. This opposes the traditional view that is that essence precedes existence, according to which we are seen as having a purpose, and values, all of which is determined before birth. (I personally think that the idea of essence preceding existence is rather obscure. There is no way that our values and purpose can be determined by genetic code. DNA is what we are, not who we are. ) Existentialists pose another question to the opposition: how can we be happy in a world devoid of significance and meaning? The loss of external values allows us to derive value from within ourselves. This value is greater than any imposed by outside forces, and thus cannot be taken away from said sources. Although many human beings live a life devoid of true happiness, it does not mean that we are incapable of attaining such a goal. If a person finds the meaning for their life, whether it be one meaning, or many, there is a large chance that they can achieve such a utopia without recognizing outside influences. People of different viewpoints follow different methods, as well as believe in different forces that shape our nature. It is in my opinion, as well as that of the existentialist, that we shape our own nature through decisions and actions made by our own free will; that we are ultimately free to act independently from the influences of outside forces. That we can ultimately ignore the views imposed on us, and achieve a sustainable level of happiness in the process. Human nature is a frail thing; people are just looking for someone else to blame for their actions and for who they are, even when there isn’t any. Therefore, there is no one to blame for who we are, but ourselves.

Unforgiven essays

Unforgiven essays In 1992, actor/director Clint Eastwood produced a Western called Unforgiven. He also directed and starred in the movie, receiving an Academy Award for the former and a nomination for the latter. The movie itself won the coveted Best Picture Award that year. It was a bit out of the norm for an Oscar winner, bleak and remorseless without the heroism and justice with which moviegoers have grown accustomed. It lacked the happy ending that makes people leave the theater beaming or, in some cases, even teary-eyed. What it was, however, was a brilliantly directed and edited glimpse of what the Old West might have really been like. There are no good guys or bad guys, only ordinary people with ordinary weaknesses. The title of the film undoubtedly refers to more than one man or one crime of violence (which the movie opens with) against a woman. This is a world without forgiveness, where so-called good and bad people are alike; all pulled back into some fundamental sin. The plot for Unforgiven centers on William Munny (Clint Eastwood), a former murderer and thief who, for the love of a good woman, gave up his former life to raise a family and make his living as a farmer. With his wife now dead and his farm failing, Will is reluctantly drawn back into his old ways by the Schofield Kid (Jaimz Woolvett), a self-proclaimed killer who brings Munny word of a bounty being offered in the Wyoming town of Big Whisky. The bounty is on the heads of two cowboys who slashed the face of prostitute there, the womans co-workers have offered a reward for the deaths of the cowboy who did the cutting and his accomplice. Will initially declines the Kids offer, but later reevaluates his situation and teams up with his old partner in crime, Ned Logan, and they set off to join the Kid. The excursion will pit him against William Little Bill Dagget (Gene Hackman), the authoritarian sheriff of Big Whisky, as...

Why the Spanish Verb Estar Is Used With Muerto

Why the Spanish Verb Estar Is Used With Muerto Finding the reason why estar is used instead of ser in sentences like mi padre est muerto is probably to be found somewhere in the history of the Spanish language rather than in any logical application of the rules of grammar. To the native Spanish speaker, ser and estar are two separate verbs, seldom interchangeable. But because they can both be translated as to be, they have been the source of confusion over the years to English speakers learning Spanish as a second language. Estar vs. Ser If grammar were only a matter of following rules, one could make good arguments for using either ser or estar. Rather than list opposing arguments (which would probably serve more to confuse than anything else), here are two related rules that make a good case for using estar. First is that when a form of ser is followed by a past participle, it generally refers to the process of a verbs action taking place, while estar followed by a participle generally refers to a completed action. For example, in los coches fueron rotos por los estudiantes (the cars were broken by the students), fueron rotos passively refers to the action of the cars being broken. But in los coches estaban rotos (the cars were broken), the cars had previously been broken. Similarly, the use of estar generally suggests there has been a change. For example, tà º eres feliz (you are happy) suggests the person is by nature happy, while tà º ests feliz (you are happy) suggests that the persons happiness represents a change from a previous state. Following either of these guidelines for choosing the right to be would result in the use of a form of estar in a sentence such as Mi padre est muerto. One might also come up with arguments for using ser, and ser is often the choice incorrectly made by beginning Spanish students. But the fact is that estar is used with muerto, and it is also used with vivo (alive): Mi padre est muerto; mi madre est viva. (My father is dead; my mother is alive.) All logic aside, the indisputable rule that estar is the verb of choice with muerto is just something youll have to remember. Thats just the way it is. And after a while, estar is the verb that will sound right.

Carnivorous Plants

Carnivorous Plants Carnivorous plants are plants that capture, kill, and digest animal organisms. Like all plants, carnivorous plants are capable of photosynthesis. Since they usually live in areas where the soil quality is poor, they must supplement their diet with nutrients gained from digesting animals. Like other flowering plants, carnivorous plants use tricks to entice insects. These plants have developed specialized leaves that work to lure and then trap unsuspecting insects. Key Takeaways Carnivorous plants are plants that have the ability to eat animal organisms. These highly specialized plants are able to both lure and trap insects.The Venus flytrap (Dionaea muscipula) is the most well known of the carnivorous plants. They live in wet areas such as bogs and swamps.Sundews are covered in tentacles. Their tentacles make a sticky dew-like substance that attracts insects.Bladderworts are plants that dont have roots and are often found in aquatic areas and in areas with wet soil. They capture insects via a trapdoor.Other examples of carnivorous plants include tropical pitcher plants and North American pitcher plants. There are several genera of carnivorous plants and hundreds of carnivorous plant species. Here are some of my favorite genera of carnivorous plants: Flytraps - Dionaea muscipula Dionaea muscipula, also known as the Venus flytrap, is probably the most well known of the carnivorous plants. Insects are lured into the mouth-like leaves by nectar. Once an insect enters the trap it touches tiny hairs on the leaves. This sends impulses through the plant triggering the leaves to close. Glands located in the leaves release enzymes that digest the prey and the nutrients are absorbed by the leaves. Flies, ants, and other bugs are not the only animals that the flytrap may snare. Frogs and other small vertebrates may sometimes become trapped by the plant as well. Venus flytraps live in wet, nutrient-poor environments, such as bogs, wet savannas, and swamps. Sundews - Drosera Sundew feeding on a green lacewing. Reinhard Dirscherl/WaterFrame/Getty Images Plus Species of plants from the genus Drosera are called Sundews. These plants live in wet biomes, including marshes, bogs, and swamps. Sundews are covered with tentacles that produce a sticky dew-like substance that glitters in the sunlight. Insects and other small creatures are attracted to the dew and become stuck when they land on the leaves. The tentacles then close around the insects and digestive enzymes break down the prey. Sundews typically capture flies, mosquitoes, moths, and spiders. Tropical Pitchers - Nepenthes Plant species from the genus Nepenthes are known as Tropical Pitcher plants or Monkey Cups. These plants are typically found in the tropical forests of Southeast Asia. The leaves of pitcher plants are brightly colored and shaped like pitchers. Insects are lured to the plant by the bright colors and nectar. The inside walls of the leaves are covered with waxy scales that make them very slippery. Insects may slip and fall to the bottom of the pitcher where the plant secretes digestive fluids. Large pitcher plants have been known to trap small frogs, snakes, and even birds. North American Pitchers - Sarracenia Species from the genus Sarracenia are called North American Pitcher plants. These plants inhabit grassy marshes, swamps, and other wetlands. The leaves of Sarracenia plants are also shaped like pitchers. Insects are lured to the plant by nectar and may slip from the edge of the leaves and fall to the bottom of the pitcher. In some species, the insects die when they drown in water that has accumulated at the bottom of the pitcher. They are then digested by enzymes that are released into the water. Bladderworts - Utricularia Utricularia australis (bladderwort). Paul Starosta/Corbis Documentary/Getty Images Plus Species of Utricularia are known as Bladderworts. The name comes from the tiny sacs, which resemble bladders, that are located on the stems and leaves. Bladderworts are rootless plants found in aquatic areas and in wet soil. These plants have a trapdoor mechanism for capturing prey. The sacs have a small membrane cover that acts as a door. Their oval shape creates a vacuum that sucks in tiny insects when they trigger hairs that are located around the door. Digestive enzymes are then released inside the sacs to digest the prey. Bladderworts consume aquatic invertebrates, water fleas, insect larvae, and even small fish. More About Carnivorous Plants For more information about carnivorous plants, take a look at the Carnivorous Plant Database and The Carnivorous Plant FAQ. Sources Reece, Jane B., and Neil A. Campbell. Campbell Biology. Benjamin Cummings, 2011.

Economics Project Essay Example | Topics and Well Written Essays - 1000 words

Economics Project - Essay Example He started developing the ‘dialectical materialism’ which addresses the economic order efficiency and its inherent weaknesses and contractions that lead to its own decay/ collapse. The concept attempted to critic capitalism tenets and prediction of its own collapse. His constant revolt against the current rulers saw him exiled in Belguim, France and Britain where he eventually died in London (Heilbroner, 136 - 139). Economic Ideas and concepts Karl Marx is often referred to as the father of Socialism and Communism owing to his Marxist theories and The Communist Manifesto. He was a critique of capitalism which dominating Europe by them after overthrowing feudalism. According to Marx, current tenets of capitalism were not sustainable as they had little regard for laborers who are important in the production process. While critiquing the capitalism, Marx argued that while capitalism was revolutionary, industrializing and driven by universal qualities of development, it expl oited and alienated laborer resulting to high unemployment rate. As a result, the system was subjects to depression shocks and the exploitation led to suffering of most laborer. These conditions were the recipes that would lead to class struggle, system decay and eventual collapse of capitalism. In essence therefore, what Marx was suggesting is a form of production process that would give decency to the working class. Such a method of production would be socialism or what is referred to as inexorable system of Karl Marx’ (Heilbroner 167). His assertions make sense when viewed in the light of modern capitalism as modern practices have come to recognize the importance of labor as a factor of production. Most companies are concerned about the welfare of their staff where business objectives are being driven by triple bottom line. Such an approach will most likely reduce tension between owners of capital and laborers. Karl Marx also contributed immensely to labor relations and in dustrial actions. He viewed the society as comprising of two classes i.e. the owners of capital and laborers who provided labor. According to him there exists a class struggle as each wants to maximize from each other. Unfortunately, the owners of capital who often forms part of the ruling class oppress the workers through long working hours and poor pay. Given the power of the ruling class and increased population, are able to maximize value at the expense of the laborers. Welfare of the workers is not a priority, maximizing surplus value and profit is. Heilbroner (32) noted that the English ruling class ‘failed to understand what fluid and mobile labour force would seek to achieve’. According to Marx, Capitalists optimize the discrepancy between labor markets and produce market to make surplus value. Surplus value is realized when input costs are lower than output price and the surplus according to Marx is taken away from laborer due the imbalance. The surplus value a rises from unpaid work as they are only paid enough to keep the body and soul together i.e. to make laborers survive. He noted that in the long run as class struggle continues, the surplus value reduces considerably a situation which forces the owners of capital to rescue their capital. In labor relations, the argument makes sense in that most laborers will go on

The only role women play in war is that of the innocent victim Essay

The only role women play in war is that of the innocent victim - Essay Example Thesis statement: The comparison based on Mother Courage and Her Children by Bertolt Brecht and Women of Troy by Euripides proves that the only role women play in war is that of the innocent victims. Comparison: A. Role of women in Thirty Years' War of 1618–1648 and Trojan War First of all, one can easily identify that Brecht was totally against Fascism and Nazism. So, he made use of the context of German invasion of Poland (1939) as the plot of his play, Mother Courage and Her Children. But he infused the plot to another context, i.e., the Thirty Years' War. The plot revolves around the life history of Anna Fierling (Mother Courage) and her futile attempt to make a living by serving as a canteen woman. Besides, Mother Courage considers her job as an opportunity to help her family. Bloom makes clear that â€Å"In the opening scene, Brecht uses â€Å"the song of Mother Courage† to project an attitude- Anna Fierling’s response to war as an arena for commerce† ( 37). From a different angle, her involvement in the Thirty Years' War (say, her service in Swedish Army) was indirect but she was forced to face its after effects (the death of her children) in a direct way. Besides, her daughter Catherine was killed during the war, without any solid reason. On the other side, in the play Women of Troy, the playwright portrays the women characters as the victims of the Trojan War. Their status in the society as the members of the royal family does not help them to be safe from the after effects of the war. For instance, the women of Troy faced multiple problems due to the utter defeat in the war with the Greek warriors. Besides, the women characters were not responsible for the war but they were forced to undergo a number of problems. Salisbury states that â€Å"In the Women of Troy, at the end of the war the women of Troy are enslaved, and Euripides shows the horror of warfare through their eyes† (153). Almost all the women characters in the play, like Hecuba, Helen, Andromache and Cassandra were not directly involved in the war. But abduction of Helen was the grass root level reason behind the war. To be specific, the women characters in Play Women of Troy were the victims of the after effects of the Trojan War. B. Family crisis One can easily identify that Brecht makes use of the main female characters in the play Mother Courage and Her Children, as his mouthpiece to communicate with the viewers. For instance, Brecht makes use of the character Anna Fierling and her service in the Swedish Army to unveil the futility of war. Besides, Anna Fierling’s service did not help her to save her children from the after effects of the dreadful war. Instead, her children (Eilif, Kattrin, and Swiss Cheese) were killed during the war. But Carney opines that† When Mother Courage lose one of her children, her immediate reaction is businesslike: it is necessary to divide up the work anew† (101). Her attempt to se rve the Swedish Army in the Thirty Years' War and to save her family became unsuccessful. Her daughter Kattrin (Catherine) was one of the direct victims of the war. Besides, Yvette Pottier, a female character in the play, was a prostitute who used to entertain the soldiers. One can easily identify that these female characters (say, Anna Fierling, Catherine and Yvette Pottier) were not directly involved in the war, but were forced to be the direct victims. On the other side, in the play Women of Troy, the women charac

Stress Essay Example | Topics and Well Written Essays - 250 words - 1

Stress - Essay Example This kind of stress is experienced equally by administrators, managers, employees and workers. Segal et al (2008, para.2) assert that â€Å"while some stress is a normal part of life, excessive stress interferes with your productivity and reduces your physical and emotional health, so it’s important to find ways to keep it under control†. A little stress is beneficial, as it keeps one going and forces him to maintain his good performance. This is called positive stress. However, negative stress weighs heavily on one’s well being because one feels weakened enough to fight the hurdles (Volhard & Volhard, 2011). Behavioral effects of stress include discontentment, argumentative behavior, lack of attentiveness, lack of commitment to the job, and conflicts in relationships (Soreq, Friedman, & Kaufer, 2009, p.42). Physiological effects include headache, stomachache, slow digestion, obesity, decreased sex drive, blood pressure leading to coronary heart diseases, and var ious skin infections (Mayo Clinic, 2011). Before treating stress, it is important to rule out the possibility of factors that accompany stress. Segal, J., Horwitz, L., Jaffe-Gill, E., Smith, M., & Segal, R. (2008). How to reduce and manage job and workplace stress. Stress at Work. Retrieved 6 April, 2012, from

Community Paper Research Example | Topics and Well Written Essays - 750 words

Community - Research Paper Example 524 8,186,453 8,829,383 281,421,906 293,045,739 Source: http://oasis.state.ga.us/oasis/qryPopulation.aspx Population by RACE 2004 The newer books might have these listed as non-Hispanic, White etc. Feel free to change. (Newton) County Black Hispanic White Native Americans Asian Other # % # % # % # % # % # % 24,451 22.08 2,376 1.87 55,312 74.20 183 0.21 903 0.72 70 0.11 State of Georgia Black Hispanic White Native Americans Asian Other # % # % # % # % # % # % 2,612,936 28.48 598,322 5.32 5,862,978 62.65 27,457 0.22 229,741 2.10 11275 0.14 United States Black Hispanic White Native Americans Asian Other # % # % # % # % # % # % 2,349,542 28.7 35,305,818 12.55 194,552,774 69.13 2,068,883 0.74 10,123,169 3.60 467,770 0.17 LEVEL OF EDUCATION Age 25 + High School 13,601 34.75 1,486,006 28.65 52,168,981 28.63 Associates 1,610 4.11 269,740 5.20 11,512,833 6.32 Bachelors 3,715 9.49 829,873 16.00 28,317,792 15.54 Graduate 1,977 5.05 430,305 8.30 16,144,813 8.86 Source: http://quickfacts.census.g ov INCOME BY HOUSEHOLD (Newton) County Georgia United States Year 2000 2000 2000 # % # % # % 200,000 308 1.40 70,843 2.36 2,502,675 2.37 MEDIAN INCOME (Newton) County Georgia United States 2000 44,875 41,901 41,990 Source: http://quickfacts.census.gov HEALTH STATISTICS Legend: T=Total W=White AA=African American O=Other Ethnic Group Use the following two forms for Mortality and Morbidity rates that are specific to your target population. _ (Deaths and percent of deaths; SIDS) ____________MORTALITY RATE per 100,000 population ***Change years for your data if necessary!!!!** Georgia (Newton) County T W AA T W AA 2000 113 44 69 2 0 2 2001 112 60 51 1 0 1 2002 141 78 59 4 2 2 2003 104 50 54 2 1 1 2004 130 68 59 1 0 1 (Source: For US, Health United States, Statistical Abstract of the US, [will need several years], MMWR, Prevention Profile.) For GA/County, use Georgia Vital Statistics for the year requested. _ (Sexually transmitted disease cases and rate) ____________ MORBIDITY RATE per 1 00,000 populations Georgia (Newton) County T W AA T W AA 2000 52,642 5,006 30,015 216 38 124 2001 53,258 4,795 25,479 296 47 147 2002 55,347 5,916 31,868 347 62 175 2003 55,624 6,239 30,802 380 63 202 2004 52,388 5,277 26,340 371 42 157 (Source: For US, Health United States, MMWR, Prevention Profile, Local and State Health Departments, CDC) The focus of this paper is on AIDS as a community health issue in Newton County, the state of Georgia in the United States. There lacks a representative national system of surveillance of the prevalence of AIDS infections (Prachakul, Grant and Keltner,

Knowledge management Assignment Example | Topics and Well Written Essays - 2000 words

Knowledge management - Assignment Example Companies which have been able to enjoy the competitive advantage have been as a result of effective implementation of knowledge management within its workforce. Knowledge management has been effective to the companies in increasing their performances since it comes with a number of benefits to the company which makes it more efficient for the companies. Knowledge management encompasses many strategies which are used in identifying, creating, representing and enabling the employees to better understand their roles. This research paper will focus on how DHL international has used knowledge management in improving its services as well as the challenges it met during its implementation. DHL DHL is a logistical and communication company offering services all across the globe. The company provides its customers with easy to use services and products which are of innovative and provide solutions ranging from dialogue marketing to supply of industrial materials in the supply chain. DHL Comp any services include Shipping, Tracking, Export services, Import services, Domestic services as well as providing small business solutions and industry solutions. The company operates in more than 200 countries around the world among them being USA, Germany, England, and Kenya among other countries. In efforts to ensure that the company renders its services effectively to the clients, the company has employed different professionals which have been efficient in putting the company at the competitive advantage every year in. The company has a workforce of over 470, 000 employees worldwide which makes it one of the largest top employing companies in the world. The company also manages over one million customers per hour of those who seeks its operations (DHL, 2012). Since the inception of the company in 1969, the company has been steadily increasing in its performances over the years as a result of its innovations in the workforce. Even with the upswing and global economic turmoil, DH L increased its revenues in the first half of the 2011 which was also witnessed in the second quarter. As a global logistical company, the company has been benefiting through increasing its trade volumes with increasing revenues each year among its divisions. The improved profitability of the Company has been achieved through the companies restructuring measures which were implemented in the previous years. Additionally, the companies consolidated revenues increased by 3.5% on the second half the year 2011 to stand at more than 25, 681 Euros. The company has outreach programs through Corporate Social Responsibility program which ensures it maintains its relationship with the customers who are part of the society as well as introducing new products to them (DHL, 2012). Challenges in Implementing Knowledge Management Implementing knowledge managements has been a challenge to many organizations as a result of many reasons. It demands a lot which organizations are not just willing to of fer immediately though they are willing to enjoy the benefits of its implementation. DHL is one of the companies which have been successful as a result of its implementation though it faced a lot of challenges for it to be finally incorporated in the organization. The challenges which were met by DHL are discussed below. One of the challenges that the organization experienced was developing the culture in the organizati

Summative Assignment (Business Plan Essay Example | Topics and Well Written Essays - 750 words

Summative Assignment (Business Plan - Essay Example The vast majority of our lodgings were among the first structures to be raised in adolescent urban area. Few inns can brag that their groups actually grew up around them. Today, we have added present day downtown area properties to our gathering, with center areas that permit you to join the rushing about of the city, make part in the move of the business locale, and appreciate the society, the lights and qualities of road life. We promise consistency all through our gathering of lodgings and resorts by holding fast entirely too broad measures. Focal buying guarantees the same great pleasantries are accessible to all visitors wherever they visit. All these and more make each Oasis in an uncommon spot, and your stay, an exceptional experience (Software, 2015) â€Å"Transforming minutes into memories for our visitors† is the mission statement of Oasis hotel and resorts. On the off chance that anything positively characterizes Oasis Hotels & Resorts, it is the quality we append to enduring memories. Expanding upon a century of experience as hoteliers has blessed us with a rich convention of neighborliness. We offer encounters that are legitimately nearby, in inns and resorts of unrivaled vicinity. Furthermore real accommodation is attained to when captivating administration and meticulousness hoist each one stay into valued memory. We realize that even the best areas and offerings would be negligible without extraordinary visitor administration. Our talented and persuaded staff is furnished with the instruments and the attitude to commonly convey on this guarantee. Inside an Oasis experience, each visitor is offered a warm welcome and is made to feel uncommon, esteemed and increased in value. We transport our visitors to exceptional spots saturated with special structural engineering, expressive dà ©cor and wonderful imaginativeness. Oasis areas dont simply ooze history; a lot of people are nothing short

Knowledge management Assignment Example | Topics and Well Written Essays - 2000 words

Knowledge management - Assignment Example Companies which have been able to enjoy the competitive advantage have been as a result of effective implementation of knowledge management within its workforce. Knowledge management has been effective to the companies in increasing their performances since it comes with a number of benefits to the company which makes it more efficient for the companies. Knowledge management encompasses many strategies which are used in identifying, creating, representing and enabling the employees to better understand their roles. This research paper will focus on how DHL international has used knowledge management in improving its services as well as the challenges it met during its implementation. DHL DHL is a logistical and communication company offering services all across the globe. The company provides its customers with easy to use services and products which are of innovative and provide solutions ranging from dialogue marketing to supply of industrial materials in the supply chain. DHL Comp any services include Shipping, Tracking, Export services, Import services, Domestic services as well as providing small business solutions and industry solutions. The company operates in more than 200 countries around the world among them being USA, Germany, England, and Kenya among other countries. In efforts to ensure that the company renders its services effectively to the clients, the company has employed different professionals which have been efficient in putting the company at the competitive advantage every year in. The company has a workforce of over 470, 000 employees worldwide which makes it one of the largest top employing companies in the world. The company also manages over one million customers per hour of those who seeks its operations (DHL, 2012). Since the inception of the company in 1969, the company has been steadily increasing in its performances over the years as a result of its innovations in the workforce. Even with the upswing and global economic turmoil, DH L increased its revenues in the first half of the 2011 which was also witnessed in the second quarter. As a global logistical company, the company has been benefiting through increasing its trade volumes with increasing revenues each year among its divisions. The improved profitability of the Company has been achieved through the companies restructuring measures which were implemented in the previous years. Additionally, the companies consolidated revenues increased by 3.5% on the second half the year 2011 to stand at more than 25, 681 Euros. The company has outreach programs through Corporate Social Responsibility program which ensures it maintains its relationship with the customers who are part of the society as well as introducing new products to them (DHL, 2012). Challenges in Implementing Knowledge Management Implementing knowledge managements has been a challenge to many organizations as a result of many reasons. It demands a lot which organizations are not just willing to of fer immediately though they are willing to enjoy the benefits of its implementation. DHL is one of the companies which have been successful as a result of its implementation though it faced a lot of challenges for it to be finally incorporated in the organization. The challenges which were met by DHL are discussed below. One of the challenges that the organization experienced was developing the culture in the organizati

Analysing the measure likelihood of success on the merits Assignment

Analysing the measure likelihood of success on the merits - Assignment Example In addition, the applicant’s attorney should prove to the court that effort has been made to stop to notify the adverse party and should give the reasons to maintain the claim that no court notice is required or the adverse party. The case of Ben Njoroge Muchunu & 2 others v Phares Muchunu & 2 others [2013] eKLR. In case, the applicants sought an order of temporary restraint to prohibit the plaintiff and the defendant against issuing the share of $52,000 as the proceeds from the sale of an estate to the late Benjamin Njoroge Wamanja. The applicants claimed that the money way held in the banks account in the names of the defendants’ and the plaintiffs’ affidavit. The basis of success was the fact that the applicants did not face any objection in their claim since there was no affidavit to deny the applicants claim. Therefore, in cases of denial of the issuance of a court injunction the applicants were entitled to suffer permanent damages that neither the defendant nor the plaintiff would compensate the applicant (Henry 47). The other alternative available is for the plaintiff and the defendant to establish to the court how they would compensate the applicants. In NEW YORK TIMES V. SULLIVAN, 376 U.S. 254, 84 S. Ct. 710, 11 L. Ed. 2d 686 [1964]. The New York times made a detailed advertisement titled â€Å"Heed their rising Voices† in a bid to raise funds to protect martin Luther King Jr against an Albama denunciation of false swearing. The Alabama court denied the application of temporary restraining order of defamation of Montogery Safety commissioner, L.B. Sullivan who was the police supervisor. The court claimed that the advertisement made by the New York Times did not mention Sullivan hence the commissioner had no base to sue the press for defamation. Also, these facts are evident in American Chicle Co. v. Topps Chewing Gum, Inc., 208 F.2d 560, 563 (2d Cir. 1953) (L. Hand, J.). The failure of the plaintiff was as a result of lack o f evidence of defamation hence the press could not be restrained from exercising their freedom of speech. In the case of Data General Corp. v. Digital Computer Controls, Inc 1971. The data general corporation exposed design information through its Nova 1200 minicomputer informing the owners of its secrecy of the design drawings in a contract agreement. Digital computer controls designed its minicomputer that resembled that of digital General Corporation that had sued in court to restrain digital computer controls against the use of heir technology illegally. The court help that a digital computer controls had breached the secrecy rights of the digital general corporation (Patterson 334). The bases for success of the digital general corporation case against digital computer controls were the fact that digital general corporation had applied sufficient security for their drawings hence digital computer controls breached the contractual terms by using confidential information for perso nal gain. In Carlill v. Carbolic Smoke Ball Co. [1893] Q.B. 256 (C.A.). the carbolic smoke ball company placed a newspaper advertisement promising 100 pounds to any person who consumed smoke balls three times in a day as directed by the company. Carlill consumed the smoke balls as directed and contracted influenza. She claimed the reward from the company, but the company denounced any contractual obligation with Carlill. The court applied temporary

Expression of Recombinant Green Fluorescent Protein (rGFP)

Expression of Recombinant Green Fluorescent Protein (rGFP) Expression and Purification of recombinant Green Fluorescent Protein (rGFP) from E. coli using Ni2+-Agarose Column Chromatography. Andrea Bustamante Janakikeerthika Darmarpandi Abstract Green Fluorescent Proteins are vital components of bioluminescence in marine animals. There unique ability to withstand and recover from harsh conditions and regain fluorescence was of great interest. The purpose of the following set of experiments was to express and purify a His6-Xpress epitope tagged recombinant form of Green Fluorescent Protein grown and harvested from E. coli. The desired protein is initially released into solution using the properties of freeze-quick thaw cycles that then help release the contents of the nucleus of neighboring bacteria following a chain reaction. It is then submitted through a Ni2+-agarose affinity chromatography column where the target protein was purified. The resulting wash and elution fractions where run through a Bradford assay, SDS-PAGE/Coomassie blue staining, and a Western blot to determine the molecular weight of the protein to be 32kDa. The overall specific activity was determined to be 433000 RFU/ mg of total protein with a resulting 20 percent purity. The results show that expression and purification of rGFP from bacterial cells was possible. Introduction Aequorea victoria is a jellyfish capable of producing a green fluorescent light when Ca2+ ions activate a photoprotein, known as aequorin, which excites Green Fluorescent Protein (GFP). Wild type GFP is a 27kDa, homodimer composed of 238 amino acid residues that absorbs light at an excitation wavelength of 395nm (blue light) and emits light at an emission wavelength of 510nm (green light). Aequorea victoria GFP has a distinctive three dimensional structure that encases a chromophore (formed by cyclization of Ser65-dehydrogenized Tyr-Gly67) and allows for stability under harsh conditions (Prasher, 229-230.) . This structure allows for regaining of fluorescence even after the protein has been denatured upon removal of the denaturant. Therefore, GFP’s are extremely stable to changes in pH, temperature, oxidation and reduction, and chemical reagents (Pan, Pickett, and Rippel 225.) Poly-histidine tags involve addition of a series of histidine residues to the N or C terminus of a protein of interest. Poly-histidine tags are affinity tags that serve to facilitate protein purification by exploiting the positively charged histidine residue’s affinity for negatively charged columns. This series of experiments involved a six repeat histidine codon contained within a DNA plasmid which resulted in a recombinant Green Fluorescent Protein that contained a six residue histidine tag located at the N-terminus. The His ­6 tagged recombinant Green Fluorescent Protein was then subjected to Ni2+-agarose column affinity chromatography. Ni2+-agarose affinity chromatography allows for the purification of poly-histidine tagged proteins due to the selectivity and affinity of the Ni2+-agarose matrix for His6 tagged proteins. rGFP binds the column due to the interactions between the His6 tagged proteins in the mobile phase with the metal Ni2+ ions immobilized within the matrix in the stationary phase. The Ni2+ ions contained within the matrix are capable of binding electron rich molecules including histidine residues and allowing most other molecules to pass unbound. This results in the binding of the desired protein to the column and the purging of most undesired proteins and contaminants from the column into wash fractions (Ninfa, et al. 100-101.) The column was then subjected to imidazole, which competes with rGFP for Ni2+ ion attachment, and this allows for the elution of the target protein. Due to its unique properties, isolation of GFP was of great interest and expression and purification were the main focus of the following series of experiments. A suitable way to accomplish this was devised using the combination of poly-histidine tagging and affinity chromatography. The purpose of this experiment was to express and purify a six-Histidine tagged recombinant form of Green Fluorescent Protein from E. coli through the use of Ni2+-agarose affinity chromatography. After expression and purification, a Bradford assay was performed to estimate total protein amount. This was followed by SDS-PAGE/Coomassie blue staining to determine purity and molecular weight. The confirmation of the presence of rGFP was done using the Western Blot. Materials and Methods Growth of G strain In a test tube, 10ml of liquid LB growth media containing 100ug/ml Amp and 25ug/ml Cam was inoculated with a single bacterial colony of strain G (BL21(DE3)uv>) and was allowed to grow overnight at 37 °C. The culture was shaken until saturated. In a flask, 500ml of liquid LB media (pre-warmed to 30 °C) was inoculated with about 4 ml of the saturated overnight culture (or until the 500ml culture reached an OD600 reading of 0.1) and allowed to grow at 37 °C until the OD600 reading reached 0.5. At approximately OD600 ~0.5, or time zero, 1ml of the culture was harvested into a 1.5ml centrifuge tube and pelleted. The supernatant was discarded and the â€Å"G0† pellet stored at -20 °C for later use. The remaining culture was induced with 1mM IPTG and allowed to grow. After 3 hours, 1ml of the culture was harvested into a 1.5ml centrifuge tube and pelleted. The supernatant was discarded and the â€Å"G3† pellet stored at -20 °C for later use. An additional 15ml of the IPTG induced culture was harvested into a 15ml centrifuge tube and pelleted. The supernatant was discarded and the â€Å"G3-15ml† was stored at -20 °C. Preparation of rGFP Crude Extract Immediately after removal of the â€Å"G3-15ml† pellet from freezer, breaking buffer [10mM Tris, pH 8.0; 150mM NaCl] was added into the centrifuge tube. The breaking buffer was pipetted up and down (being careful not to introduce air) until pellet had thawed and homogeneity was reached. The solution was transferred into a 1.5ml centrifuge tube, vortexed for 5 minutes, labeled and placed in 37 °C water bath for 10minutes after which the centrifuge tube was transferred to a rotating platform shaker in a dry air 37 °C incubator for 20 minutes. After lysis, the mixture was centrifuged at 14000xg, 4 °C, for 10 minutes. In a dark room in the presence of a hand held UV light, the fluorescence of the pellet and supernatant where observed the recorded. The supernatant was then decanted and care was taken not to get the pellet back into the supernatant as centrifugation would be required if this did occur. This supernatant was the GCE (rGFP crude extract) Preparation of Ni2+-agarose Column In a 3ml plastic syringe, enough glass wool was placed into the well to cover up to the 1/4 ml marking. The syringe was secured onto a ring stand and placed perpendicular to the ground. About 100ul of breaking buffer was pipetted into the top of a closed luer-lock and allowed to overflow. 1ml of buffer was then pipetted into the syringe column and the luer-lock was immediately screwed onto the syringe. An additional 2ml of breaking buffer was added to the column and several drops of buffer were allowed to flow out. The luer-lock was then returned to the closed position. A total of 500ul of breaking buffer was added to the column and then 1ml of a 0.5ml bed volume Ni2+-agarose slurry was added to the column. The luer-lock was opened and agarose matrix was allowed to â€Å"gravity pack.† The column was pre-equilibrated with 5ml of breaking buffer and then the luer-lock was returned to the closed position. Ni2+-NTA Chromatography Separation Procedures 100ul of GCE was transferred into a centrifuge tube, labeled, and set aside. Breaking buffer was added to remaining GCE if content was less than 1ml. GCE was slowly applied to the Ni2+-agarose column and allowed about 5-10 minutes for protein to bind to column. The luer-lock was opened and 0.5ml of effluent was collected into 1.5ml centrifuge tube and labeled W1. This was repeated with the subsequent effluent labeled W2.The column was then observed under an ultraviolet light and fluorescence recorded. The column was then washed with 4ml of buffer in 0.5ml increments. The effluent was collected and labeled W3 to W10. The column was then washed again with a total of 5ml of breaking buffer. This effluent was discarded. A total of 5ml of elution buffer containing 10mM Tris, pH 8.0; 150mM NaCl, 300mM imidazole was added to the column in 0.5ml increments. The eluents were collected and labeled E1-E10.The column was then observed under a UV light and the fluorescence recorded. The W1-W6 and E1-E6 fractions were also observed under UV light and their fluorescence rec orded qualitatively. Determining Total Protein Amount A standard curve was created using six different samples of Bovine Serum Albumin (1mg/ml) of known amount. The amounts of BSA used all had a final volume of 50ul and included 0ug, 3ug, 5ug, 10ug, and 20ug total proteins. A total of 1ml of Bradford reagent was added to each, vortexed, and allowed to incubate for 10 minutes. The results where read using 200ul in a microtiter dish and read using a microplate reader set to 595nm. The results where plotted on a graph as absorbance (595nm) vs. BSA (ug) and a best fit line was drawn. The Bradford assay was then performed once on the W1-W6 and E1-E6 samples. Any samples whose absorbance fell outside the standard curve were repeated less sample in the assay. Once all samples fell within the standard curve, the Bradford assay was repeated two more times for each sample. The total protein amount was then extrapolated from the standard curve using the absorbance values. Estimating Purity and Molecular Weight The SDS-PAGE was prepared using a 12 percent resolving gel that was poured between the Bio-Rad glass plate â€Å"sandwich† and allowed to polymerize. A 5 percent stacking gel was prepared and added on top of the resolving gel, a comb was inserted, and the gel was allowed to polymerize. Once that polymerized, the combs were removed and the electrophoresis tank was set up. 15ul of G0, G3, GCE, W3, W4, E2, and E3 samples were added to the SDS-PAGE along with a standard molecular weight ladder. The samples were electrophoresed at 200volts for 45 minutes. The gel was then stained using Coomassie blue dye and the stain removed. Confirmation of rGFP 2-ÃŽ ²-mercaptoethanol was added to the centrifuge tubes containing the G0, G3, GCE, W3, W4, E2, and E3 samples and were loaded along with a molecular weight ladder and electrophoresed as described above. The stacker was removed and the resulting gel set up for transfer onto a nitrocellulose membrane for Western Blot analysis. The overall setup required a â€Å"building up† of components with the positive electrode base on the bottom, followed by filter paper soaked in transfer buffer, nitrocellulose paper above that, the SDS/PAGE layer, another layer of filter paper soaked in transfer buffer, Western blot solution was poured over all the components, and finally the negative electrode lid was locked into position. To ensure transfer, the nitrocellulose gel was stained using Ponceau S and allowed to incubate for two minutes on a rocker and then destained using ddH2O. The membrane was then blocked using 5% non-fat dry milk/TBS solution and incubated for 30 minutes on a rocking p latform. This was then and washed three times with 0.05%Tween 20/TBS with 5 minutes of incubation between each wash. It was then probed with mouse IgG anti-Xpress epitope MAb solution and allowed to incubate for 45 minutes. The 0.05%Tween 20/TBS wash was repeated in triplicate. A secondary probe using sheep IgG anti-mouse IgG conjugated horseradish peroxidase polyclonal anti-serum solution was performed as above and then washed in triplicate. The nitrocellulose gel was developed using TMB until desired intensity was reached and development was stopped with water and results recorded immediately. Results The expression of the target protein was doubly repressed in the G0 (uninduced) sample of E. coli. First, the Lac repressor protein binds to the lac operator and prevents transcription by T7 RNA polymerase (Garrett and Grisham 915-916). Second, T7 RNA was repressed by lysozyme protein that binds to T7 RNA polymerase and inhibits transcription. Expression of rGFP in the G3 (3 hour post induction) sample was made possible through the use of IPTG (Garrett and Grisham 914.) The purpose of IPTG was to repress the Lac repressor which resulted in T7 RNA polymerase being able to transcribe DNA downstream of the T7 promoter and expression of His6-Xpress-GFPuv, resulting in the fluorescent capable recombinant Green Fluorescent Protein. (Figure 1) This resulting recombinant GFP is a 279 amino acid protein. rGFP has a six Histidine tag at its N terminus between amino acids 5 and 10, an Xpress epitope between amino acids 24 and 31, Green Fluorescent Protein between amino acids 39 and 277, and a 3 amino acid end tag between amino acids 277 and 279. The chromophore is found between amino acids 103 and 105 in the DNA sequence. (Figure 2) Results of Ni2+-agarose affinity chromatography and Bradford assay indicated that the E3 (elution 3) sample contained the most rGFP activity with approximately 18,600 RFU (relative fluorescent units) and an estimate 43ug of total protein. The specific activity calculated for the sample was 433000 RFU/ mg of total protein. (Figure 3) The SDS-PAGE/Coomassie staining gave an estimate molecular weight for rGFP of 32kDa based on a total traveled distance of 2.3cm along the SDS/PAGE. The overall purity of the band was approximately 20 percent. The higher molecular weight band was most likely contaminants at about 45kDa and the lower molecular weight band was possibly a result of the degradation of the c-terminus at 27kDa. (Figure 4) Western Blot indicated prominent bands in the E3, E2, GCE, and G3 lanes. Lanes W4 and W3 showed very light bands and lane G0 shows an absence of bands. All visible bands appear at about 32 kDa and therefore confirm the presence of rGFP. (Figure 5) Conclusion The successful expression and purification of recombinant Green Fluorescent Protein is significant in the scientific community due to the possible uses for it in the future. Green Fluorescent Protein is significant because it provides an inexpensive and relatively easy method of detection. The possibility for real time detection means result could be obtained in real time. Future experiments will focus on linking rGFP to proteins during transcription and translation. This would result in a desired protein with a GFP tag whose fluorescence can then be used for identification. This should result in the ability to locate a target protein using the fluorescence of rGFP. Future applications of GFP could include incorporation into the genetic code of small mammals. These could encode fluorescent neurons which in turn could help further research in areas such as nerve tissue regeneration or other advances in neurobiology. Its unique properties of endurance could be exploited to understand how it can endure harsh environments and still regain functionality after remediation. This would have significant applications in molecular and cellular biology in understanding cellular degeneration and how help patients with diseases involving cellular degeneration. Bibliography Pan, Jing, Elizabeth Pickett, and Scott Rippel. Biochemistry Laboratory Lecture Notes. Dallas: UTD copy center, 2013. 225-289. Print. Pan, Jing, Elizabeth Pickett, and Scott Rippel. Biochemistry Laboratory Manual. Dallas: UTD copy center, 2013. 38-77. Print. Prasher, Douglas C., Virginia K. Eckenrode, et al. Primary Structure of the Aequorea victoria green-fluorescent protein. Gene. 111. (1992): 229-233. Print. Garrett, R., and Charles M. Grisham. Biochemistry. 4th ed. Belmont, CA: Brooks/Cole, Cengage Learning, 2010. Print. Ninfa, Alexander J., and David P. Ballou. Fundamental laboratory approaches for biochemistry and biotechnology. Bethesda, Md.: Fitzgerald Science Press, 1998. 89-107. Print.

Sickle Cell Anemia :: essays research papers fc

The problem is that sickle cell anemia affects about 72,000 Americans in the United States. Sickle cell anemia is an inherited disease in which the body is unable to produce normal hemoglobin, an iron-containing protein. Abnormal hemoglobin can morph cells that can become lodged in narrow blood vessels, blocking oxygen from reaching organs and tissues. The effects of sickle cell anemia are bouts of extreme pain, infectious, fever, jaundice, stroke, slow growth, organ, and failure. Sickle cell anemia hurts many people today in fact it hurts about 72,000 Americans. But some doctors are finding cures for this inherited disease. This disease causes mainly strokes and fever. With this disease a stroke is not predictable, a stroke can happen as early as a one month old as a baby. It can hurt a person really bad because it causes them to not be able to do many things like can't play sports, and things that gets your heart pumping because if the red blood cells gets clogged up it can causes a stroke because oxygen can't flow. Most Americans who have sickle cell anemia are of African descent. The disease also affects Americans from the Caribbean, Central America, and parts of South America, Turkey, Greece, Italy, the Middle East and East India. Since sickle cell anemia is an inherited disease if both parents have the trait for sickle cell, their baby's chances of having sickle cell disease is one in four. Many doctors are trying to find cures for this disease by trying the solution on patients. Doctors at Emory University and University of Mississippi Medical Center in Jackson, Chicago. Doctors in Emory University in Atlanta credited an experimental stem cell transplant that for the first time is not from a related donor. This transplant cured the inherited disease from Keone Penn who is 13 years old from Georgia. He suffered a stroke at 5 years old and had a fever of 106 degrees, "I almost died" (Ferraro, Newspaper Article) What the doctors did was replaced the boy's bone marrow with stem cells taken from the umbilical cord blood of an infant not related to him. Dr. Ruby Bellevue of New York Methodist Hospital in Brooklyn has patient that he wants to do the transplant procedure on, but he is waiting for more studies to come out to see what the long-term effects are. Some effects could be rejection, complications, and/or death.